RARγ is required for correct deposition and removal of Suz12 and H2A.Z in embryonic stem cells.

TitleRARγ is required for correct deposition and removal of Suz12 and H2A.Z in embryonic stem cells.
Publication TypeJournal Article
Year of Publication2011
AuthorsAmat R, Gudas LJ
JournalJ Cell Physiol
Volume226
Issue2
Pagination293-8
Date Published2011 Feb
ISSN1097-4652
KeywordsAnimals, CCAAT-Enhancer-Binding Proteins, Cell Differentiation, Cells, Cultured, Cytochrome P-450 Enzyme System, Embryonic Stem Cells, Epigenesis, Genetic, Gene Expression Regulation, Histones, Mice, Mice, Knockout, Polycomb Repressive Complex 2, Promoter Regions, Genetic, Receptors, Retinoic Acid, Repressor Proteins, Retinoic Acid 4-Hydroxylase, Tretinoin
Abstract

Retinoic acid (RA) induces embryonic stem cell differentiation. The effects of RA are mediated by retinoic acid receptors (RARs) that promote epigenetic changes controlling gene transcription. We show here that RARγ, in the absence of the ligand RA, is required for deposition of the histone variant H2A.Z and the polycomb group protein Suz12 at RA target genes, and that in embryonic stem cells both RARγ and Suz12 exist in a multi-protein complex in the absence of ligand. Addition of RA causes removal of H2A.Z and Suz12 from RARγ target genes when the genes are transcriptionally activated.

DOI10.1002/jcp.22420
Alternate JournalJ. Cell. Physiol.
PubMed ID20857416
PubMed Central IDPMC3369573
Grant ListR01 CA043796 / CA / NCI NIH HHS / United States
R01 CA043796-21 / CA / NCI NIH HHS / United States