The Effect of Ethanol Consumption on Composition and Morphology of Femur Cortical Bone in Wild-Type and ALDH2*2-Homozygous Mice.

TitleThe Effect of Ethanol Consumption on Composition and Morphology of Femur Cortical Bone in Wild-Type and ALDH2*2-Homozygous Mice.
Publication TypeJournal Article
Year of Publication2021
AuthorsMalkovskiy AV, Van Wassenhove LD, Goltsev Y, Osei-Sarfo K, Chen C-H, Efron B, Gudas LJ, Mochly-Rosen D, Rajadas J
JournalCalcif Tissue Int
Volume108
Issue2
Pagination265-276
Date Published2021 Feb
ISSN1432-0827
Abstract

ALDH2 inactivating mutation (ALDH2*2) is the most abundant mutation leading to bone morphological aberration. Osteoporosis has long been associated with changes in bone biomaterial in elderly populations. Such changes can be exacerbated with elevated ethanol consumption and in subjects with impaired ethanol metabolism, such as carriers of aldehyde dehydrogenase 2 (ALDH2)-deficient gene, ALDH2*2. So far, little is known about bone compositional changes besides a decrease in mineralization. Raman spectroscopic imaging has been utilized to study the changes in overall composition of C57BL/6 female femur bone sections, as well as in compound spatial distribution. Raman maps of bone sections were analyzed using multilinear regression with these four isolated components, resulting in maps of their relative distribution. A 15-week treatment of both wild-type (WT) and ALDH2*2/*2 mice with 20% ethanol in the drinking water resulted in a significantly lower mineral content (p < 0.05) in the bones. There was no significant change in mineral and collagen content due to the mutation alone (p > 0.4). Highly localized islets of elongated adipose tissue were observed on most maps. Elevated fat content was found in ALDH2*2 knock-in mice consuming ethanol (p < 0.0001) and this effect appeared cumulative. This work conclusively demonstrates that that osteocytes in femurs of older female mice accumulate fat, as has been previously theorized, and that fat accumulation is likely modulated by levels of acetaldehyde, the ethanol metabolite.

DOI10.1007/s00223-020-00769-1
Alternate JournalCalcif Tissue Int
PubMed ID33068139
PubMed Central IDPMC8092984
Grant ListR01 AA018332 / AA / NIAAA NIH HHS / United States
R01 AA011147 / AA / NIAAA NIH HHS / United States
R37 AA011147 / AA / NIAAA NIH HHS / United States
T32 DK098132 / DK / NIDDK NIH HHS / United States
R37 AA11147 / / Foundation for the National Institutes of Health /
T32 GM089626 / GM / NIGMS NIH HHS / United States
RO1 AA18332 / / Foundation for the National Institutes of Health /