Title | Activation of HIF2α in kidney proximal tubule cells causes abnormal glycogen deposition but not tumorigenesis. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Fu L, Wang G, Shevchuk MM, Nanus DM, Gudas LJ |
Journal | Cancer Res |
Volume | 73 |
Issue | 9 |
Pagination | 2916-25 |
Date Published | 2013 May 01 |
ISSN | 1538-7445 |
Keywords | Animals, Basic Helix-Loop-Helix Transcription Factors, Carcinoma, Renal Cell, Cell Proliferation, Cell Transformation, Neoplastic, Gene Expression Regulation, Neoplastic, Glycogen, Humans, Hypoxia-Inducible Factor 1, alpha Subunit, Kidney, Kidney Neoplasms, Kidney Tubules, Mice, Mice, Inbred C57BL, Mice, Transgenic, Mutation |
Abstract | Renal cell carcinoma (RCC) is the most common primary cancer arising from the kidney in adults, with clear cell renal cell carcinoma (ccRCC) representing approximately 75% of all RCCs. Increased expression of the hypoxia-induced factors-1α (HIF1α) and HIF2α has been suggested as a pivotal step in ccRCC carcinogenesis, but this has not been thoroughly tested. Here, we report that expression of a constitutively activated form of HIF2α (P405A, P530A, and N851A, named as HIF2αM3) in the proximal tubules of mice is not sufficient to promote ccRCC by itself, nor does it enhance HIF1αM3 oncogenesis when coexpressed with constitutively active HIF1αM3. Neoplastic transformation in kidneys was not detected at up to 33 months of age, nor was increased expression of Ki67 (MKI67), γH2AX (H2AFX), or CD70 observed. Furthermore, the genome-wide transcriptome of the transgenic kidneys does not resemble human ccRCC. We conclude that a constitutively active HIF2α is not sufficient to cause neoplastic transformation of proximal tubules, arguing against the idea that HIF2α activation is critical for ccRCC tumorigenesis. |
DOI | 10.1158/0008-5472.CAN-12-3983 |
Alternate Journal | Cancer Res. |
PubMed ID | 23447580 |
PubMed Central ID | PMC3642229 |
Grant List | R25 CA105012 / CA / NCI NIH HHS / United States T32 CA062948 / CA / NCI NIH HHS / United States R25 25105012 / / PHS HHS / United States |